EVALUATION OF DISRUPTION METHODS FOR RELEASING INTRACELLULAR PRODUCT FROM COCCUS AND BACILLUS TYPE BACTERIAL CELLS

Abstract

A comparative evaluation of four different cell-disruption methods of releasing intracellular products from Neisseria denitrificans and Nocardia corallina was investigated. The cell disruption methods evaluated in this study were high-pressure homogenization (HPH), batch mode bead milling (BM) and ultrasonication (US). US and traditional construction HPH were determined to be cell-geometry-dependent methods, less effectively disrupting the Nocardia corallina bacilus in comparison with the Neisseria denitrificans coccus. However, bead mill was found to be less effective in the disruption the Neisseria denitrificans of coccus. Microfluidizer, opposedsteam HPH, was found to be a cell-type-independent method with the lowest energy consumption and highest disruption rates among the analyzed methods. Microscopic and size-distribution analyses revealed the mechanism of disruption to differ between cells. Coccus are totally disrupted and lose their native structure after disruption, while bacillis are just broken but retain their geometrical structure which is truncated enough to release the intracellular products.

DOI: http://dx.doi.org/10.5755/j01.ct.59.1.1531