Antimicrobial Efficiency of Photoactivated Chlorophyllin-Chitosan Complex

Abstract

The aim of this study was to evaluate antimicrobial efficiency of photoactivated chlorophyllin-chitozan complex against food pathogen Salmonella enterica.

Salmonella enterica was used for experiments. Aliquots of bacterial suspensions (~1×10⁷ CFU/ml in 0.9 % NaCl) with 0.001 % chlorophyllin-0.1 % chitosan complex (Chl-CHS) were incubated in the dark at 37 °C for 0–120 min. For the photoactivation, the samples were exposed to light (λ = 405 nm) for 30 min (light dose 17.3 J/cm²). Microbial viability was evaluated by spread plate method.

Chl-based photosensitization treatment reduced the bacterial population by 1.39 log. Extremely high antibacterial efficiency was demonstrated after photoactivation of Chl-CHS complex (7.3 log reduction of microbial population).

Experimental data support the idea that photoactivated CHS-Chl complex in slightly acidic environment can be a useful tool against Gram (-) bacteria S. enterica..

DOI: http://dx.doi.org/10.5755/j01.ct.61.3.2710